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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C/-80˚C
- 保质期:
24months
- 英文名:
Recombinant Salmonella heidelberg (strain SL476) D-serine dehydratase
- 库存:
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- 规格:
详询
Host Species:E.coli
Target Name:SDHD Uniprot ID: B4TA53 Tag Info:His-SUMO-tag 产品详细说明请点击:Recombinant Salmonella heidelberg (strain SL476) D-serine dehydratase风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Expression and Preparation of Fusion Proteins from Recombinant gt11 Phages
by the fusion of the foreign sequence to the carboxyl-terminus of β-galactosidase in λgt11 expression system. The conventional method for preparing fusion proteins from the recombinant λgt11 clones involves production of phage lysogens in Escherichia coli strain
Expression and Preparation of Fusion Proteins from Recombinant gt1.1 Phages
in Escherichia coli strain Y1089 followed by inducing lac Z-directed fusion protein expression with isopropyl-β-D -thiogalactopyranoside (IPTG) (1 ). This method has two limitations: it is time-consuming, and phage lysogeny occurs at a low frequency
; shaded portions in the figure),so that the fusion proteins are expressed from a T7 promoter upon induction with isopropylthio--D-galactoside (IPTG).E.coli strain BL21(DE3)cells (Novagen)carrying the fusion plasmids were inoculated into 300 ml (IPIII
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