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- 美国沃特世Waters
- 美国
- 186000848
- 2025年07月16日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
blue tetrazolium in 70% dimethylformamide, 30% H2O. Store at -200 C in a glass container covered with foil. Does not freeze at this temperature. Levamisole (100X): 50 mM in H20. Store at -200 C. PMS (100X): 2 mg/ml phenazine methosulfate
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