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上海希言科学仪器有限公司
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文献和实验7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column- Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol.)0.75M KCl+ Buffer C (188x106cells) Thawed on ice. Pellet 20min.in microfuge
.)0.75M KCl Buffer C (188x106cells) Thawed on ice. Pellet 20min.in microfuge at 4℃. Saved super - but noted pellet not tight and some got into super. Froze - 80℃. Thawed sample Pelleted 20 min.in microfuge - rest of pellet came down.@4.0ml
PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
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