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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
will run through the column quickly. This step can be performed during antibody incubation (Step 12). 18. Apply cell suspension onto the column: load ~250-300 million cells per column. Allow the cells to pass through and collect
(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume: 1.5 ml 1M Hepes 1.05ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 24.15ml H20 ChIP high salt buffer 30ml final volume: 1.5ml 1M Hepes
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
is to be processed on an AX-500 cartridge then use the prescribed 12 ml of S1, S2, and S3 for the cartridge. On the other hand, if a 500 ml BAC culture is to be processed on an AX-500 cartridge then at least 20 ml ({500 ml / 100 ml} * 4) of each of the three buffers
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