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文献和实验centrifuge to collect all drops from walls of tube. Overlay the sample with half volume of mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid. Place samples in a thermocycler
. See Enhancers & Inhibitors in this Guide.· Enhancers needed. Some reactions may amplify only in the presence of additives. See Enhancers & Inhibitors in thisGuide.· Mineral oil problem. The reaction must be overlaid with highquality, nuclease-free light mineral
in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min
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