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文献和实验the cerebral hemispheres. Fix 1/2 of brain in formalin and store for several weeks; then drain off excess formalin and repackage in a heat-sealed plastic bag (x2) or airtight plastic container and FEDEX to the repository, billing recipient
Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
, from testing. The plasma bag is emptied by cutting the long tube with a pair of sharp scissors(be careful not to splash). To the plasma (300mL) is added 3mL of 100mM EDTA (sterile filtered) to a final concentration of 1mM. Protease Inhibitors may also be added
-hot polyclonal, or 1:30 dilution of tissue culture supernatant for a good monoclonal. Do the incubation in a sealameal bag for 1 hr at room temp on a rocker. Can go overnight in the fridge. Remember: it's critical to do a control with no 1° antibody
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