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文献和实验Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1. Assemble the glass plates and spacers (0.75 mm thick). 2. Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3. Seal with 1 ml
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
. We will verify the concentration of your concentrated DNA sample and adjust it for microinjection to 0.5 to 1.0 ng per ul. DNA Storage: Store resuspended DNA at 4C. Buffer Storage S1 - 50 mM Tris-HCl, 10 mM EDTA, 100 µg RNAse A / ml, pH
Method for Single-Cell Electroporation
-10 µm Eppindorf Pipettor tip, or a 1 cc plastic insulin syringe that had been melted over flame and pulled to a long fine tip. Circuit setup: A thin silver wire (diameter 0.25mm) is inserted into the micropipette touching the DNA solution
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