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- 2025年07月06日
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文献和实验Amicon Stirred Ultrafiltration Cells
line. Operation Place cell on magnetic stirring table. Connect inlet line to regulated gas pressure source (Nitrogen gas is recommended for pressurizing cell! Use of compressed air can cause large pH shifts due to dissolution
or distilled water.将凝胶浆体一次性倒入。沿抵着柱壁的玻棒倒入凝胶浆可以减少混入气泡。在储液器装入洗脱液或蒸馏水。 7. Screw on the reservoir cap tightly and connect it to the pump.拧紧贮液器盖，与泵连接。 8. Open the bottom outlet of the column and start the pump at the flow given for step 1in Table 2. 打开底部的出口，将泵以表
. Have the tubing and connections ready to connect the column in tandem with a G25 column (2.5 x 44 cm), also equilibrated in Buffer G, but don''t have them connected yet. The tubing between the outflow of the DNaseI column and the inlet of the G25 column
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