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文献和实验Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries
Determining in vivo substrates phosphorylated by different mitogen-activated protein kinases (MAPKs) is critical to understanding how these enzymes regulate cell division, differentiation, and growth. The evolution of new technologies
Determination of Epitopes by Mass Spectrometry
bromide (CNBr)-activated Sepharose beads. Digestion of the antigen with endoproteinase LysC resulted in an affinity-bound peptide whose fine structure was characterized by digestion with carboxypeptidase Y and aminopeptidase M. All steps of this method
Microwave Processing Techniques for Microscopy
of epoxy-resin embedded material is the most widely used method for TEM investigations of biological material. As in histoprocessing techniques, the cells or tissues are first fixed, then dehydrated and embedded. (The epoxy resins are miscible with alcohol
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