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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
can be made. For the introduction of randomized X positions in the peptide sequences, take the appropriate Fmoc-AA-mix stock solution from the freezer, bring to room temperature, and activate by adding DIC (1.5 µl per 100 µl-vial
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
16 µM streptavidin–Alexa Fluor 532 conjugate (see recipe ) 5 mM free D‐biotin (see recipe ) PBS‐TBN
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