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上海希言科学仪器有限公司
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文献和实验Liquid Chromatography‐Mass Spectrometry Analysis of DNA Polymerase Reaction Products
Acquity BEH octadecylsilane (C18) UPLC column (1.7 µm particle size, 1.0 mm × 100 mm; Waters Associates) Oligo Composition Calculator v1.2 software (http://library.med.utah.edu
Site-directed hydroxy radical mapping of nucleosome positions in vitro
all on a pre-run 160x200x1mm, 5% (1:40) acrylamide, 0.2xTBE native gel equilibrated at 4°C. The gel is both pre-run and run at 300V for 180-240 minutes. When the sample contains larger amounts of nucleosome it may be necessary to increase the amount of ferrous
in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using
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