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上海希言科学仪器有限公司
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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
in methanol.iii. Pack the column with 4 cm of 5 µm strong cation-exchange material in methanol. 10. Connect the column to an Upchurch PEEK MicroCross (for details, see Analysis of Complex Protein Mixtures Using Nano-LC Coupled
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
EDTA‐plasma samples with C4A3 and C4B1 allotypes, or with any other known C4 allotypes of interest 10 µg/µl carboxypeptidase B (Sigma) in 0.1 M NaCl
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