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上海希言科学仪器有限公司
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文献和实验Mag-Bind® mRNA Protocol (Standard Protocol)
as this increases the possibility of mRNA degradation and RNase contamination 3) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 1.5 mL of RNA-Solv ® Reagent to a 3.5 cm diameter dish, and passing the cell lysate several times
Vybrant® DyeCycle Green and Orange Stains
Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO
ES / MEF cell culture and electroporation of targeting construct
ul cold 1 X Hebs to your pelleted cells. Place a sterile BTX cuvette (2 mm gap) in a 630 A Safety Chamber and attach the electrodes securely to the Electro Cell Manipulator 600. Pipette the cells up and down with a 5 ml pipette, and add 800 ul
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