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- 文献和实验
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- 供应商:
上海希言科学仪器有限公司
- 规格:
1000个/箱
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文献和实验from the cultures. Place the sample in a separate test tube (it need not be sterile). 2. Dilute 4 parts of stock Trypan Blue with 1 part of 5X saline and add 0.1 ml of the diluted dye to your sample. Mix gently. 3. Set up a hemocytometer and cover slip
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
coating with polymer or adding a layer of metal. Place the slide into a sterile rectangular four-chamber cell culture vessel (or use tissue culture slide flask if polystyrene surface is desired). Count cells and seed 1.5 x 106 cells onto the slide in 5 ml
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