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文献和实验Selection of Human Immunoglobulin Light Chains from a Phage-Display Library
may be a pathogenetic factor in asthma. With the aim of investigating the possible cause-effect relationship between autoantibodies to VIP and asthma, we have cloned catalytic antibody light (L) chains from the immune repertoire of an exercise-induced asthma patient
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides
Figure 4.5.1 Generic oligonucleotide 3′‐conjugate. View Image
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