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- American Research Products
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- 2025年07月11日
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文献和实验Protein Carbonyl Determination Using Biotin Hydrazide
Protein oxidation is a recognized component of aging and a consequence of severe or prolonged exposure to reactive oxygen species (ROS). Direct attack of protein by ROS causes formation of protein-bound carbonyl groups (1 ). These carbonyl
(or saved as input) and the beads (putatively bound with the siRNA/Flag complexes) washed 3 to 5 times with 2x wash buffer (note 2);6.Following the final wash the bound siRNA/protein complexes are eluted from the avidin-biotin bound beads by incubation
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
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