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- American Research Products
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- 2025年12月27日
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文献和实验Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules
The advent of soluble MHC multimer technology has allowed for the flow-cytometric direct identification of specific-MHC restricted antigen-specific T cells in mixed cell populations and also enabled the direct phenotyping and cloning
To determine the immunogenicity of vaccine candidates, it is essential to establish an accurate and convenient methodology for assessing the level of antigen-specific T-cell responses, and several methodologies have been developed
Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
into insoluble inclusion bodies, and cell death, thus affecting the amount of soluble protein that can be obtained from E. coli grown in culture. To take full advantage of a variety of strategies developed to improve the expression of soluble protein in E. coli
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