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文献和实验Isolation of High-Molecular-Weight DNA from Animal Cells
such methods for all preparations of DNA from mammalian cells. The first three methods described below are derived from that of Blin and Stafford (5 ) and should yield high-mol-wt (HMW) DNA from solid tissues, blood, or cells in culture suitable
The Isolation of High Molecular Weight Eukaryotic DNA
The isolation of high molecular weight eukaryotic DNA in good yield is an important prerequisite for the analysis of specific sequences by Southern blotting (Chapter 9 ), or for molecular cloning in phage or cosmid vectors (Chapter 49 ).
High Molecular Weight DNA Extraction from Arabidopsis
Large DNA molecules (>l00 kb) are extremely sensitive to mechanical shearing m aqueous solution. Therefore, classical DNA extractron procedures from living tissues generally do not allow recovery of DNA fragments larger than 50‐100 kb m size
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