GNB1, 1-340aa, Human, His tag,

去Tag不留痕――NEB原核表达系列

科学家的杰出贡献而首次在美国本土以外设立分厂.顺应国内市场的要求，NEB中国专门为国内市场提供特别小包装――常规小包装的一半（反正他家小包装的量也超大的），这样单个成本就便宜多啦。NEB出品的原核表达有两类融合表达系统，pMAL™是久经考验的可溶性表达系统，以麦芽糖结合蛋白为融合表达框架的蛋白融合表达纯化系统，另一个就是刚发布时震动整个原核表达界的IMPACT（Intein Mediated Purification with an Affinity Chitin-binding Tag）系统，一个不需

Purification of Human Multiprotein Complexes using OneSTrEP Technology

. For more theoretical background and a protocol for purification of recombinant Strep -tag® fusion proteins from E.coli please refer to Schmidt and Skerra (2007). In comparison to other commonly used purification strategies (i.e. HA and FLAG double-tag

Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)

complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone