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- 2025年07月10日
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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology
. For more theoretical background and a protocol for purification of recombinant Strep -tag® fusion proteins from E.coli please refer to Schmidt and Skerra (2007). In comparison to other commonly used purification strategies (i.e. HA and FLAG double-tag
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone
PROTEOMICS 4 (1): 56-72 JAN 2005 Times Cited: 9 10. Qian WJ, Monroe ME, Liu T, et al. Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using O-16/O-18 labeling and the accurate mass and time tag approach
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