
Taq-Plus DNA Polymerase
- ¥120 - 4500
- 常规PCR
- 江苏连云港
- EG15109/G/X/N/GN/XN
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 供应商:
江苏愚公生命科技有限公司
- 规格:
500 U/5,000 U/8 x 2500 U/500 U/5,000 U/8 x 2500 U
| 规格: | 500 U | 产品价格: | ¥120.0 |
|---|---|---|---|
| 规格: | 5,000 U | 产品价格: | ¥900.0 |
| 规格: | 8 x 2500 U | 产品价格: | ¥3400.0 |
| 规格: | 500 U | 产品价格: | ¥150.0 |
| 规格: | 5,000 U | 产品价格: | ¥1200.0 |
| 规格: | 8 x 2500 U | 产品价格: | ¥4500.0 |
通用PCR酶
Taq-Plus DNA Polymerase
| Cat No. |
规格 |
价格 |
说明书 |
| EG15109 | 500 U | 120 元 | |
| EG15109G |
5,000 U | 900 元 | |
| EG15109X |
8 x 2500 U | 3,400元 | |
| EG15109N |
500 U | 150 元 |
|
| EG15109GN |
5,000 U | 1,200元 | |
| EG15109XN | 8 x 2500 U | 4,500元 |
| EG15109 | EG15109G | EG15109X | |
| Taq-Plus DNA Polymerase (5 U/μl) |
100 μl |
2 x 500 μl |
8 x 500 μl |
| 10X PCR Buffer for Taq |
2 x 1.25 ml |
20 x 1.25 ml |
80 x 1.25 ml |
| EG15109N | EG15109GN | EG15109XN | |
| Taq-Plus DNA Polymerase (5 U/μl) |
100 μl |
2 x 500 μl |
8 x 500 μl |
| 10X PCR Buffer for Taq | 2 x 1.25 ml | 20 x 1.25 ml | 80 x 1.25 ml |
| dNTP, 2.5 mM each | 800 μl | ||
| dNTP, 10mM each | 2 x 1 ml | 8 x 1 ml |
保存:-20ºC
Taq-Plus DNA Polymerase是从嗜热菌Thermus aquaticus中开发的一种重组高热稳定DNA聚合酶,比普通Taq酶热稳定性更好。本产品含有5'→3'外切酶活性,不包含3'→5'外切酶活性,适合5 kb以内DNA片段扩增。同时具有脱氧核苷酸转移酶活性,会在PCR产物的3'末端添加一个额外的腺苷酸,产物可直接用于TA克隆。
PCR扩增;PCR鉴定、菌落PCR等快速扩增。
本产品的PCR产物不推荐用于聚丙烯酰胺凝胶电泳。
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文献和实验Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
and combined with diluted Taq DNA polymerase and the individual fluorescent end-labeled universal primers (see Appendix C) to yield the final reaction mixes, that are sufficient for 24 template samples. Once the above mixes are prepared, four aliquots
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration
We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
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