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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Cyclin E Polyclonal Antibody Cy5.5 Conjugated
- 靶点:
Cyclin E
- 浓度:
1mg ml
- 应用范围:
IF(IHC-P)
- 宿主:
Rabbit
- 适应物种:
Human
- 标记物:
Cy5.5
- 克隆性:
Polyclonal
- 保存条件:
Store at 4C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human Cyclin E
- 规格:
100ul
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文献和实验Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy5
Production of Polyclonal Antisera
All immunochemical procedures require a suitable antiserum or monoclonal antibody raised against the antigen of interest. Polyclonal antibodies are raised by injecting an immunogen into an animal and, after an appropriate time, collecting
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