The antiserum was produced against synthesized non-phosphopeptide derived from human PLCγ1 around the phosphorylation site of tyrosine 783 (G-F-Y<sup>P</sup>-V-E).
Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PLCγ1 (Ab-783) antibody (OAEC00477, Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody (OAEC00101, Lane 3 and 4).