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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein of Human APIP
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q96GX9
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB,IHC;ELISA:1:500-1:5000,WB:1:200-1:1000,IHC:1:25-1:100
- 浓度:
>95%,Antigen affinity purification
- 靶点:
APIP
- 抗体英文名:
APIP Antibody
- 抗体名:
Probable methylthioribulose-1-phosphate dehydratase antibody
- 规格:
100μl/50μl
| 规格: | 100μl | 产品价格: | ¥1980.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥1100.0 |
保存缓冲液
-20℃, pH7.4 PBS, 0.05% NaN3, 40% Glycerol功能
APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury.The protein highly expressed in skeletal muscle and heart and binds to the CARD of Apaf-1 in competition with caspase-9. Exogenous APIP inhibits cytochrome c-induced activation of caspase-3 and caspase-9, and suppresses cell death triggered by mitochondrial apoptotic stimuli through inhibiting the downstream activity of cytochrome c released from mitochondria.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Sandwich ELISA The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act
Protein G Purification of Antibodies
onto the column. Was with 5 bed volumes of 20 mM Phosphate Buffer, pH 7.0 Elute the antibody with 1-3 bed volumes of 0.1M Glycine, collecting fractions into tubes containing 1M Tris
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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