CHEK2抗体CHEK2一抗抗体Serine/threoni
ne-protein kinase Chk2 antibody抗体Phospho-CHEK2 (T387) Antibody抗体- ￥880 - 1320
- CUSABIO
- CN
- CSB-PA006787
- 2025年07月07日
- WB, IHC, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
- Rabbit
- Human,Mouse,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from Human Chk2 around the phosphorylation site of T387.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性：
/
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse,Rat
- 保质期：
6个月
- 抗原来源：
Homo sapiens (Human)
- 目录编号：
O96017
- 级别：
优
- 库存：
200
- 供应商：
武汉华美生物工程有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA; WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
- 浓度：
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点：
CHEK2
- 抗体英文名：
Phospho-CHEK2 (T387) Antibody
- 抗体名：
Serine/threonine-protein kinase Chk2 antibody
- 规格：
100μg/50μg
| 规格： | 100μg | 产品价格： | ￥1320.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥880.0 |
保存缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.功能
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文献和实验Establishment of a Cell-Free System to Study the Activation of Chk2
The checkpoint kinase Chk2 is activated in response to DNA damage through pathways requiring protein kinases ATM and/or ATR. The means by which Chk2 is activated by these kinases still remains to be addressed. Here we describe a cell-free
from the control or treated cells; (2) preparing substrate; (3) immunoprecipitating CHK1 protein from the cells; (4) assembling the kinase assay; and (5) analyzing the phosphorylated level of the substrates by CHK1. Besides CHK1, CHK2 is another important
High-Throughput Kinase Assay Based on Surface Plasmon Resonance
part and a phospho-detection part on a sensor chip. Not only biotinylated peptides but also recombinant proteins fused with FLAG-GST tandem tag can be used as native substrates. The substrate is selectively captured by a capture antibody immobilized on a sensor chip
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