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2ml柱床体积/5ml柱床体积
| 规格: | 2ml柱床体积 | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 5ml柱床体积 | 产品价格: | ¥2500.0 |
| 产品编号 | PH-5 |
| 英文名称 | Protein G Sepharose 4 Fast Flow |
| 中文名称 | 重组蛋白G亲和层析柱 |
| 保存条件 | Store at 2-8℃. |
| 背景资料 | 应用于IgG纯化,每毫升柱体积可结合IgG:人 >25mg;兔>20 mg;羊>25 mg;小鼠>6 mg;重组蛋白G与Sepharose 4B为饱和结合。 |
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文献和实验Purification of Antibodies Using Protein A-Sepharose and FPLC
at any one time. Since the antibody combining site is left free, protein A, when covalently coupled to stationary supports like agarose or Sepharose beads, provides an excellent reagent for isolating immune complexes or immunoglobulins from a crude solution
Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
Introduction Characterizing Protein‐Protein Binding Using the Flow Cytometry Protein‐Protein Interaction Assay Basic Protocol 1: Saturation Analysis of Biotin‐RGS with Alexa Fluor532‐Gαo
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