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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温,避光
- 库存:
大量
- 供应商:
上海晶抗生物工程有限公司
- CAS号:
9002-89-5
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文献和实验Denaturing Gradient Gel Electrophoresis (DGGE)
, the denaturants used are heat (a constant temperature of 60 degrees C) and a fixed ratio of formamide (ranging from 0-40%) and urea (ranging from 0-7 M). The position in the gradient where a domain of a DNA fragment melts and thus nearly stops migrating
for enhancing protein thermostability. J . Mol. Biol. 290, 595-604. 8. Vogt, G. , Woell, S. , and Argos, P. (1 997) Protein thermal stability, hydrogen bonds, and ion pairs. J . Mol. Biol. 26 9, 631-643. 9. Szilagyi, A. and Zavodszky,
Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
This protocol is for four time-points in 12-well plates. Scale up or down as needed. Day 1: Plate cells: Split one or more 10-cm plate(s) with 80-100% confluent HeLa Tet-off cells to a final volume of 10-11 ml. Make sure
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