大鼠PPAR-γ基因干扰慢病毒实验服务

PPAR-γ

名称: 过氧化物酶体增殖因子活化受体γ

描述: Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. Defects in PPAR gamma can lead to type 2 insulin-resistant diabetes and hyptertension and could play a role in the genetic predisposition to obesity

参考文献: # Muscle-specific PPARγ-deficient mice develop increased adiposity and insulin resistance but respond to thiazolidinediones. J Clin Invest. 2003 August 15; 112(4): 608–618

病毒转染实验是研究目的基因、目的蛋白功能极其有效的分子生物学方法， 其原理是构建过表达或者iRNA干扰病毒质粒，根据实验需要，分别包装成腺病毒、慢病毒、逆转录病毒，感染宿主细胞或动物组织，调控宿主细胞或动物组织相应基 因表达及蛋白合成，为了解目的基因及蛋白对细胞功能的调控提供了有利的手段，广泛受到科研工作者重视及青睐，成为分 子机制研究不可缺少的重要实验手段。

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