Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of G863D, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes EGFR (G863D), but not wild type EGFR from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Western blot:
Western blot analysis of recombinant EGFR (G863D) and wildtype proteins. Purified His-tagged EGFR (G863D) protein (amino acid 702-987) (Cat. #10222) and corresponding wild-type protein (Cat. #10210) were blotted with anti-EGFR (G863D) mouse monoclonal antibody (Cat. #26119). Proteins loading were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G863D) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G863D) plasmid (right column), then fixed and stained with anti-EGFR (G863D) monoclonal antibody (Cat. #26119).