Background: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
Immunogen: Recombinant full length protein of Ran
Applications: ELISA, WB, IP, IHC
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:2000 IP: 1 µg for 1~2 mg total cellular proteins IHC: 1:100 dilution
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Ran antibody recognizes Ran of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of Ran proteins. Whole-cell lysate of MEF cells was used for western blots with the monoclonal anti-Ran antibody (Cat # 26020).