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- 技术资料
- 库存：
现货
- 供应商：
上海笃玛生物科技有限公司
- 运输方式：
低温
- 规格：
株
培养基：90%高糖DMEM+10%FBS
传代方法：Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
LMH鸡肝癌细胞系 高性价比
生长条件：95%空气+5%二氧化碳 37℃
生长特性：贴壁生长
存储条件：50%高糖DMEM+40%FBS+10%DMSO 液氮
Strain：Leghorn
Karyotype：triploid; modal number = 116; six marker chromosomes
Derivation：LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.
Clinical Data：Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
Receptor Expression： estrogen receptor, positive
Tumorigenic ：Yes
Effects：Yes, forms tumors in nude mice
Year of Origin ：1981
LMH鸡肝癌细胞系 高性价比
描述 ：Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
The morphology of the cells is dendritic like.
The cells express glucose-6-phosphatase and weak canalicular ATPase activity.
The cells form tumors in athymic nude mice.
LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.
The cell line is useful for transfection studies.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.
LMH鸡肝癌细胞系 高性价比
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文献和实验AbstractAIM:To study the effect of a varying concentrations of ars enic trioxide on human hepatoma cell line BEL-7402 cultured in vitro and its mechanism of action.METHODS:The BEL-7402 cells were treated with arsenic trioxide (at the concentrations
品牌不是唯一，高性价比的荧光定量PCR仪选购指南从1996年ABI公司推出了全球第一款荧光定量PCR以来，经过10几年的发展，由于荧光定量PCR仪的在科研、检测和诊断领域的广泛应用，市场规模也不断扩大。已有多家国内外生产厂商涉足研发生产,就连Eppendorf公司也于2006年底推出了自己的荧光定量PCR仪。这些产品良莠不齐，令研究人员选择是不免挑花了眼。于是很多资金充足的实验室为了保险起见都选择购买了几家最有名气公司的产品，如ABI的7500和7900，罗氏的Lightcycler2.0
学问题。 案例一：多组学分析揭示肝癌起始细胞免疫逃逸机制和精准治疗策略 发表期刊：Cancer Cell 影响因子：44.5 发表时间：2024 年 12 月 研究疾病：肝细胞癌（Hepatocellular Carcinoma,HCC） 样本类型：患者来源的肝癌类器官（HCC organoids），肝癌细胞系（Hep3B,Huh7,PLC/PRF/5, MHCC97 H,Hep53.4）和小鼠模型（包括自发性肝癌模型和异种移植模型） 样本数量：11 个 HCC 类器官样本，4 个肝癌细胞系样本
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