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- Bioss
- bs-0294D
- 2025年10月10日
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- 规格：
1mg/10mg
| 规格： | 1mg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥6400.0 |
| 产品编号 | bs-0294D |
| 英文名称 | Donkey Anti-Goat IgG H&L |
| 中文名称 | 驴抗羊IgG H&L |
| 英文别名 | Goat Immunoglobulin G; |
| 产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:200-1000, IF, ELISA=1:2000-10000 Not yet tested in other applications. |
| 交叉反应 | Goat |
| 抗体来源 | Donkey |
| 免疫原 | Native Goat IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
| {ELISA} | {1:2000-10000} |
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文献和实验[IF={{ 6.63 }}] {Yao, Yunpeng. et al. Immunotherapy for lung cancer combining the oligodeoxynucleotides of TLR9 agonist and TGF-β2 inhibitor. CANCER IMMUNOL IMMUN. 2022 Nov;:1-18} {Other} {Mouse}
[IF={{ 5.479 }}] {Zhang J et al. Gold-platinum nanoflowers as a label and as an enzyme mimic for use in highly sensitive lateralflow immunoassays: application to detection of rabbit IgG. Mikrochim Acta. 2019 May 16;186(6):357.} {Other} {Goat}
[IF={{ 4 }}] {Song, Yuning, et al. "Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig." FEBS Open Bio (2016).} {IHC} {Goat}
[IF={{ 3.644 }}] {Teketay et al. Effect of Kisspeptin-54 immunization on performance, carcass characteristics, meat quality and safety of Yiling goats. Meat Sci . 2020 Aug;166:108139.} {ELISA} {goat}
[IF={{ 2.86 }}] {Luan, Xinhong, et al. "Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach." PLOS ONE 12.9 (2017): e0185253.} {WB} {Goat}
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
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