- ￥480 - 3600
- Bioss
- bs-0368G-PE
- 2025年10月10日
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- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥3600.0 |
| 产品编号 | bs-0368G-PE |
| 英文名称 | Goat Anti-Mouse IgM, PE conjugated |
| 中文名称 | PE标记的羊抗小鼠IgM |
| 英文别名 | Goat Anti-Mouse IgM (PE); Immunoglobulin M; |
| 产品应用 | IF=1:100-1000, Flow-Cyt=1:100-1000, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Mouse |
| 抗体来源 | Goat |
| 免疫原 | Native Mouse IgM |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
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文献和实验[IF={{ 3.24 }}] {Zhang, Yani, et al. "Selection of the Inducer for the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells In Vitro." PloS one 11.10 (2016): e0164664.} {ICC} {Mouse}
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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