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- bs-0432R-HRP
- 2025年10月10日
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100ul/1ml
| 规格： | 100ul | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1580.0 |
| 产品编号 | bs-0432R-HRP |
| 英文名称 | Rabbit Anti-Chicken IgY, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记兔抗鸡IgY抗体 |
| 英文别名 | Rabbit Anti-Chicken IgY H&L (HRP) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:500-1000 Not yet tested in other applications. |
| 交叉反应 | Chicken |
| 抗体来源 | Rabbit |
| 免疫原 | Native Chicken IgY |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | In chickens, immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally, IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG. However, in contrast to IgG, IgY does not bind to Protein A, to Protein G, or to cellular Fc receptors. Furthermore, IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem, after they were able to show differences between the immunoglobulins found in chicken eggs, and immunoglobulin G. Other synonymous names are Chicken IgG, Egg Yolk IgG, and 7S-IgG. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ELISA} | {1:500-1000} |
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文献和实验[IF={{ 5.9 }}] {Rui Bai. et al. Evaluation of the immunoprotective potential of recombinant EtMIF as a subunit vaccine candidate against Eimeria tenella infection in chickens. FRONT IMMUNOL. 2025 Jul;16:} {ELISA} {Chicken}
[IF={{ 4.02 }}] {Xu et al. A Critical Role of Bacterioferritin in Salmonella pullorum-Induced IFN-β Expression in DF-1 Cells. (2016) Front.Microbiol. 7:20} {WB} {Salmonella}
[IF={{ 2.7 }}] {Yusong Du. et al. Soybean β-Conglycinin Inhibits Broiler Growth and Nutrient Utilization by Inducing Allergic and Inflammatory Responses, Impairing Intestinal Barrier Integrity and Altering Cecal Microbiota. ANIMALS. 2025 Jan;15(12):1701} {ELISA} {Chicken}
[IF={{ 1.9 }}] {Mengyue Wang. et al. Evaluation of the Immune Effect of a Triple Vaccine Composed of Fowl Adenovirus Serotype 4 Fibre-2 Recombinant Subunit, Inactivated Avian Influenza (H9N2) Vaccine and Newcastle Disease Vaccine Against Respective Pathogenic Virus Challenge in Chickens. J APPL POULTRY RES. 2024 Jan;:100410} {ELISA} {Chicken}
[IF={{ 1.73 }}] {Liu, Dong, and Zhong-Xiang Niu. "Cloning of a gene fragment encoding chicken complement component C3d with expression and immunogenicity of Newcastle disease virus F gene?CC3d fusion protein."Avian Pathology 37.5 (2008): 477-485.i} {WB} {="Chicken"}
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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