- ￥240 - 1580
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- bs-0368G-HRP
- 2025年10月10日
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产品信息以Bioss网站为准
- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1580.0 |
| 产品编号 | bs-0368G-HRP |
| 英文名称 | Goat Anti-Mouse IgM, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的羊抗小鼠IgM |
| 英文别名 | Goat Anti-Mouse IgM (HRP); Immunoglobulin M; |
| 产品应用 | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 交叉反应 | Mouse |
| 抗体来源 | Goat |
| 免疫原 | Native Mouse IgM |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |
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文献和实验[IF={{ 9.587 }}] {Luo, Lu. et al. Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice. J NEUROINFLAMM. 2022 Dec;19(1):1-27} {WB} {Mouse}
[IF={{ 8.713 }}] {Zong-Shin Lin. et al. EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression. ELIFE. 2023 Jan} {WB} {Human}
[IF={{ 8.101 }}] {Jin Wang. et al. Irisin protects against sepsis-associated encephalopathy by suppressing ferroptosis via activation of the Nrf2/GPX4 signal axis. FREE RADICAL BIO MED. 2022 Jul;187:171} {WB} {Mouse}
[IF={{ 7.963 }}] {Xuebing Chen. et al. Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data. SCI TOTAL ENVIRON. 2022 May;:155984} {WB} {Mouse}
[IF={{ 6.953 }}] {Zi Liang. et al. β-Arrestin 2 acts an adaptor protein that facilitates viral replication in silkworm. INT J BIOL MACROMOL. Int J Biol Macromol. 2022 May;208:1009} {WB} {Escherichia Coli,Bombyx}
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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