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- bsm-0297M-AP
- 2025年10月10日
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- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥3600.0 |
| 产品编号 | bsm-0297M-AP |
| 英文名称 | Mouse Anti-Human IgG (3D3) mAb, AP conjugated |
| 中文名称 | 碱性磷酸酶（AP）标记的鼠抗人IgG H&L单克隆抗体 |
| 英文别名 | Mouse Anti-Human IgG (3D3) mAb (AP); Immunoglobulin G; |
| 产品应用 | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Native Human IgG |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
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