- ￥180 - 1200
- Bioss
- bs-0295D-HRP
- 2025年10月10日
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- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥180.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1200.0 |
| 产品编号 | bs-0295D-HRP |
| 英文名称 | Donkey Anti-Rabbit IgG H&L, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的驴抗兔IgG H&L |
| 英文别名 | Donkey Anti-Rabbit IgG H&L (HRP); Immunoglobulin G; |
| 产品应用 | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 交叉反应 | Rabbit |
| 抗体来源 | Donkey |
| 免疫原 | Native rabbit IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |
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文献和实验[IF={{ preprint }}] {Rubina Dad. et al.Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain.Research Square.} {Western blot} {Mouse}
[IF={{ 8.59 }}] {Hu et al. MARCH5 RNA promotes autophagy, migration, and invasion of ovarian cancer cells. (2017) Autophagy. 13:333-344} {IHC} {Rabbit}
[IF={{ 7.364 }}] {Wang Yujuan. et al. CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis. BMC BIOL. 2023 Dec;21(1):1-20} {WB} {Human}
[IF={{ 7.31 }}] {Ming Yang. et al. APPL1 Is a Prognostic Biomarker and Correlated with Treg Cell Infiltration via Oxygen-Consuming Metabolism in Renal Clear Cell Carcinoma. OXID MED CELL LONGEV. 2023;2023:5885203} {IHC} {Human}
[IF={{ 6.1 }}] {Song Kangping. et al. Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway. J TRANSL MED. 2024 Dec;22(1):1-19} {IHC} {Rat,Mouse}
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我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
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