- ￥240 - 1580
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- bs-0345R-FITC
- 2025年10月10日
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产品信息以Bioss网站为准
- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1580.0 |
| 产品编号 | bs-0345R-FITC |
| 英文名称 | Rabbit Anti-Human IgM, FITC conjugated |
| 中文名称 | FITC标记的兔抗人IgM |
| 英文别名 | Mouse Anti-Human IgM (FITC); Immunoglobulin M; |
| 中文别名 | 兔抗人-IgM; |
| 产品应用 | IF=1:100-1000, Flow-Cyt=1:100-1000, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | Native Human IgM |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
大多数IgM类抗体是优球蛋白不溶于水，故可用双蒸水透析纯化IgM。对那些溶于水的IgM类抗体可用饱和硫酸铵沉淀。沉淀后可以采用颗粒排斥层析法(凝胶过滤)进一步纯化。 颗粒排斥层析(size-exclusion chromatography)法又称分子筛层析或凝胶过滤，是利用微孔凝胶分离不同大小分子的抗体，可用于样品中IgG和IgM的分离，常用于对硫酸铵粗提物的进一步纯化。通过该法纯化所得抗体可用于Ig分子片段的制备、FITC、生物素或同位素标记。 一、材料和试剂
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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