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- bs-0375R-FITC
- 2025年10月10日
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- 规格：
100ul/1ml
| 规格： | 100ul | 产品价格： | ￥360.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥2400.0 |
| 产品编号 | bs-0375R-FITC |
| 英文名称 | Rabbit Anti-Guinea Pig IgM, FITC conjugated |
| 中文名称 | FITC标记的兔抗豚鼠IgM |
| 英文别名 | Rabbit Anti-Guinea Pig IgM (FITC); Immunoglobulin M; |
| 产品应用 | IF=1:100-1000, Flow-Cyt=1:100-1000, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Guinea Pig |
| 抗体来源 | Rabbit |
| 免疫原 | Native Guinea Pig IgM |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光
光素。由于FITC是小分子化合物，每一个抗体可标记几个FITC分子，IgM通常用小分子的荧光素标记，如FITC、Cy3/5、Texas Red等。 我们知道，FITC的绿色荧光强度肉眼观察已经很亮了，但是从上面的图可见，与Cy2相比还是偏弱，而DyLight 488则更胜一筹。DyLight是一种新型的荧光分子，与传统的荧光团如FITC、Rhodamine、Texas Red等相比，具有更强的荧光强度、更高的光学稳定性以及特异性等。同时，DyLight种类
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
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