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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Cucumber mosaic virus Helicase (ORF1a)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥8520.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥5268.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
ORF1aUniprot No.:
K8DRS1别名:
/种属:
Cucumber mosaic virus (cucumber mosaic cucumovirus)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
37.1 kDa表达区域:
2-302aa氨基酸序列:
MGFRGLCEAMNAHGTTILKGTMMFDGAMMFDDQGVIPELNCQWRKIRNAFSETEDATSLSGKIDSSGFTSVRKFKTMVAFDFINESTMSYIHDWENIKSFMTNQTYSYKGMTYGIERCVINAGIMTYKIIGVPGMCPPELIRHCIWFPSIKDYVGLKIPASMDLVKWKTVRVLTSTLRETEEIAMRCYNDKKAWMEQFKVILGVLSAKSSTIVINGMSMQSGERIDIDDYHYIGFAILLHTKMKYEQLGKMFDMWNASSISKWFAALTRPLRVFFSSVVRALFPTLRPREEKEFLIKLSTF蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Cucumber mosaic virus (CMV) populations in Tunisian pepper crops are mainly composed of virus reassortants with resistance-breaking properties." Ben Tamarzizt H., Montarry J., Girardot G., Fakhfakh H., Tepfer M., Jacquemond M. Submitted (JUL-2012)Function:
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文献和实验) Identification of DNA sequences required for activity ofthe cauliflower mosaic virus35s promoter. N a tu re 313, 810-812. 50. Bevan, M. W ., Mason, S. E. and Goelet, P. ( 1985) Expression of tobacco mosaic-virus coat
产生对灰质葡萄孢的抗性很有意义(Hain等,1985)。Kondo等1989年对编码水稻巯基蛋白酶抑制剂的基因组DNA做了克隆和序列分析(Kondo等,1989)。周兆斓等构建了水稻cDNA文库,分离了编码水稻巯基蛋白酶抑制剂的cDNA(周兆斓等,1996)。植物蛋白酶抑制剂是一类天然的抗虫物质,它可抑制摄食害虫对蛋白质的消化,使害虫因缺乏所需氨基酸而导致非正常发育或死亡。胡天华等人从烟草中分离出流行于我国的黄瓜花叶病毒(Cucumber Mosaic virus)(CMV),并克隆了编码该病毒外壳蛋白
酶抑制剂的基因组DNA做了克隆和序列分析(Kondo等,1989)。周兆斓等构建了水稻cDNA文库,分离了编码水稻巯基蛋白酶抑制剂的cDNA(周兆斓等,1996)。植物蛋白酶抑制剂是一类天然的抗虫物质,它可抑制摄食害虫对蛋白质的消化,使害虫因缺乏所需氨基酸而导致非正常发育或死亡。胡天华等人从烟草中分离出流行于我国的黄瓜花叶病毒(Cucumber Mosaic virus)(CMV),并克隆了编码该病毒外壳蛋白的cDNA基因(胡天华等,1989)。王春香等从感病的烟草叶片中分离纯化了马铃薯x病毒(potato
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