- ￥1180 - 2800
- Bioss
- bs-16197R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16197R |
| 英文名称 | FUT10 Rabbit pAb |
| 中文名称 | FUT10蛋白抗体 |
| 英文别名 | 3)-fucosyltransferase 10; alpha-(1; alpha-(1, 3)-fucosyltransferase 10; Fuc-TX; Fucosyltransferase 10; Fucosyltransferase X; FucT-X; fut10; FUT10_HUMAN; Galactoside 3-L-fucosyltransferase 10. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Sheep, Chimpanzee) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FUT10 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 56 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Signal Transduction > Metabolism > Amino Acids |
| 亚细胞定位 | Golgi apparatus; Golgi stack membrane. |
| 相似性 | Belongs to the glycosyltransferase 10 family. |
| 功能 | Probable fucosyltransferase. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. Hematopoietic lineages rely on Fucosyltransferases to confer a surface carbohydrate phenotype, which mediates proper cell adhesion, molecule recruitment and cell trafficking. Localized to the Golgi apparatus as a single-pass transmembrane protein, FucT-X, also designated ?(1,3)-fucosyltransferase 10 or FUT10, is a 479 amino acid protein that is involved in protein modification and glycosylation. There are seven isoforms of FucT-X that are produced as a result of alternative splicing events. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT10) Polyclonal Antibody, Unconjugated (bs-16197R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: PC-3 (human)cell Lysate at 40 ug
_x000D_ Primary: Anti- FUT10 (bs-16197R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 56 kD
_x000D_ Observed band size: 56 kD
_x000D_
_x000D_ Primary: Anti- FUT10 (bs-16197R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 56 kD
_x000D_ Observed band size: 56 kD
_x000D_
Sample:
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-FUT10 (bs-16197R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 56 kD
_x000D_ Observed band size: 56 kD
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-FUT10 (bs-16197R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 56 kD
_x000D_ Observed band size: 56 kD
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