Sample:
Testis(Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-ADAM17 (Thr735) (bs-12447R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 120 kD
- ￥1180 - 2800
- Bioss
- bs-12447R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 详细信息
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12447R |
| 英文名称 | phospho-ADAM17 (Thr735) Rabbit pAb |
| 中文名称 | 磷酸化CD156b抗体 |
| 英文别名 | ADAM17(phospho T735); p-ADAM17(phospho T735); p-CD156b(phospho T735); A disintegrin and metalloproteinase domain 17; ADAM 17; ADAM metallopeptidase domain 17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; MGC71942; Snake venom like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; Tumor Necrosis Factor alpha Converting Enzyme; A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme). |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human ADAM17 around the phosphorylation site of Thr735 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 68/91 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cancer > Invasion/microenvironment > ECM > Extracellular matrix > ADAM protein family Cancer > Signal transduction Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Metalloprotease > ADAMs Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Types of disease > Cancer Metabolism > Types of disease > Neurodegenerative disease Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease Signal Transduction > Adapters > Transmembrane Stem Cells > Signaling Pathways > Notch > Secreted |
| 亚基 | Interacts with MAD2L1, MAPK14 and MUC1. |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. |
| 相似性 | Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. |
| 功能 | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADAM17 (Thr735)) Polyclonal Antibody, Unconjugated (bs-12447R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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phospho-ADAM17 (Thr735) Rabbit pAb(bs-12447R)-50ul/100ul/200ul
￥1180 - 2800
