Sample:
Lane 1: Human U87MG cell lysates
Lane 2: Human DU145 cell lysates
Primary: Anti-phospho-ITGB3 (Tyr785) (bs-5452R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
- ￥1180 - 2800
- Bioss
- bs-5452R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
MARCH2 Rabbit pAb(bs-9336R)-50ul/100ul/200ul
￥1180
CD105 Rabbit pAb, Cy3 conjugated(bs-4609R-Cy3)-100ul
￥2980Activin A Receptor Type IC Rabbit pAb, APC-Cy5.5 conjugated(bs-5777R-APC-Cy5.5)-100ul
￥2980PIRH2 Rabbit pAb, HRP conjugated(bs-4895R-HRP)-100ul
￥2980
Anti-human CSF2Ra / GM-CSFRa / CD116 (Mavrilimumab Biosimilar)(BIO0174SM)-1mg/5mg/20mg
￥3200
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5452R |
| 英文名称 | phospho-ITGB3 (Tyr785) Rabbit pAb |
| 中文名称 | 磷酸化整合素β3/CD61抗体 |
| 英文别名 | Integrin beta 3(phospho Y785); p-Integrin beta 3(phospho Y785); Integrin beta chain, β3 precursor; Integrin beta 3; CD 61; CD61; CD61 antigen; GP3A; GPIIIa; HPA 1; HPA 4; Integrin beta 3(platelet glycoprotein IIIa antigen CD61); Integrin beta chain beta 3; ITG B3; ITGB 3; NAIT; Platelet fibrinogen receptor beta subunit; Platelet glycoprotein IIIa; platelet glycoprotein IIIa precursor; Platelet membrane glycoprotein IIIa; PTP. |
| 中文别名 | Integrin β3; Integrin-β3; Integrin β 3; Integrinβ3; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human ITGB3 around the phosphorylation site of Tyr785 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 87 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Cell cycle > Chromosome Structure > Centromere Epigenetics and Nuclear Signaling > Chromosome Structure > Centromeres Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > NFkB pathway Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Integrins > Beta Signal Transduction > Signaling Pathway > Nuclear Signaling > NFkB Pathway Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors |
| 亚基 | Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with HIV-1 Tat. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion. Interacts with MYO10. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. |
| 翻译后修饰 | Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. |
| 相似性 | Belongs to the integrin beta chain family. Contains 1 VWFA domain. |
| 功能 | Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known
技术资料暂无技术资料 索取技术资料
phospho-ITGB3 (Tyr785) Rabbit pAb(bs-5452R)-50ul/100ul/200ul
￥1180 - 2800
