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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8352R |
| 英文名称 | UBE2E3 Rabbit pAb |
| 中文名称 | 泛素蛋白连接酶E3抗体 |
| 英文别名 | Homologous to yeast UBC4/5; UB2E3_HUMAN; UBC E4; Ubc M2; UBC4/5 homolog; UBCE 4; UBCE4; UBCH 9; UBCH9; UbcM2; UBE2 E3; UBE2E3; Ubiquitin carrier protein; Ubiquitin carrier protein E3; Ubiquitin conjugating enzyme E2 23 kDa; Ubiquitin conjugating enzyme E2 E3; Ubiquitin conjugating enzyme E2E 3; Ubiquitin conjugating enzyme UBCH 9; Ubiquitin conjugating enzyme UBCH9; Ubiquitin protein ligase; Ubiquitin protein ligase E3; Ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Chicken, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human UBE2E3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ubiquitin E2 Enzymes Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Ubiquitylation Epigenetics and Nuclear Signaling > Ubiquitin & Ubiquitin Like Modifiers > E2 Ubiquitin Conjugating Enzymes |
| 亚基 | The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus (By similarity). Interacts with NEDD4L. |
| 亚细胞定位 | Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. |
| 组织特异性 | Ubiquitously expressed at low levels. Highly expressed in skeletal muscle. |
| 相似性 | Belongs to the ubiquitin-conjugating enzyme family. |
| 功能 | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 2.984 }}] {Yalin Liu. et al. UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging. Peerj. 2021 Nov;9:e12253} {WB} {Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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