Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTPRA Polyclonal Antibody, Unconjugated(bs-4154R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
- ￥1180 - 2800
- Bioss
- bs-4154R
- 2025年10月24日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4154R |
| 英文名称 | PTPRA Rabbit pAb |
| 中文名称 | 酪氨酸磷酸酶α抗体 |
| 英文别名 | HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; PTPRA; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PTPRA |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 89 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Tyrosine Phosphatases |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 相似性 | Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily. Contains 2 tyrosine-protein phosphatase domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vital cellular functions such as cell proliferation and signal transduction are regulated in part by the balance between the activities of protein tyrosine kinases (PTK) and protein tyrosine phosphatases (PTPs). The protein contains a 121 residue extracellular domain, a single transmembrane segment, and 2 tandem intracytoplasmic catalytic domains. The PTP alpha protein is ubiquitously expressed and may play a fundamental role in the physiology of all cells. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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PTPRA Rabbit pAb(bs-4154R)-50ul/100ul/200ul
￥1180 - 2800
