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phospho-Smad1/5 (Ser463 + Ser4

65) Rabbit pAb(bs-3418R)-50ul/100ul/200ul
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  • bs-3418R
  • 2025年10月24日
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3418R
    英文名称phospho-Smad1/5 (Ser463 + Ser465) Rabbit pAb
    中文名称磷酸化细胞信号转导分子Smad-1/5抗体
    英文别名Smad1(phospho S463/465); Smad1(phospho Ser463/465); p-Smad1(Ser463/465); Mothers against decapentaplegic homolog 1; BSP 1; BSP1; BSP-1; Dwarfin A; DwfA; hSMAD 1; hSMAD1; JV 41; JV4 1; JV4-1; JV41; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; MADH 1; MADH1; Madr 1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Sma and Mad related protein 1; SMAD 1; SMAD-1; SMAD family member 1; SMAD mothers against DPP homolog 1 Drosophila; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; SMAD1_HUMAN; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; Smad1; Transforming growth factor-beta-signaling protein 1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量52 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Growth factors > TGF

    Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > SMADs

    Signal Transduction > Signaling Pathway > Nuclear Signaling > SMADs

    Stem Cells > Signaling Pathways > TGF beta > Cytoplasmic

    亚基Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with SKOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome.
    亚细胞定位Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    组织特异性Ubiquitous. Highest expression seen in the heart and skeletal muscle.
    翻译后修饰Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    相似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
    功能Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (bs-3418R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (bs-3418R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    Cerebrum (Mouse) Lysate at 40 ug
    Stomach (Mouse) Lysate at 40 ug
    Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (bs-3418R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52 kD
    Observed band size: 62 kD
    产品细节图片4
    Sample:
    Lane 1: Cerebrum (Mouse) Lysate at 40 ug
    Lane 2: Heart (Mouse) Lysate at 40 ug
    Lane 3: Testis (Mouse) Lysate at 40 ug
    Lane 4: Skin (Mouse) Lysate at 40 ug
    Lane 5: Kidney (Mouse) Lysate at 40 ug
    Lane 6: Cerebrum (Rat) Lysate at 40 ug
    Lane 7: Testis (Rat) Lysate at 40 ug
    Lane 8: Skin (Rat) Lysate at 40 ug
    Lane 9: Kidney (Rat) Lysate at 40 ug
    Lane 10: Huvec (Human) Cell Lysate at 30 ug
    Lane 11: A549 (Human) Cell Lysate at 30 ug
    Lane 12: Hela (Human) Cell Lysate at 30 ug
    Lane 13: HT1080 (Human) Cell Lysate at 30 ug
    Lane 14: A431 (Human) Cell Lysate at 30 ug
    Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (bs-3418R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52 kD
    Observed band size: 54 kD

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    图标文献和实验
    该产品被引用文献

    [IF={{ 4.2 }}] {Zhang Quan-Bing. et al. Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats. EUR J MED RES. 2024 Dec;29(1):1-12} {WB} {Rat}

    [IF={{ 3.8 }}] {Xing Fuao. et al. BMP2 expression in oral squamous cell carcinoma and its effects on SCC9 cell biological behavior. SCI REP-UK. 2025 Apr;15(1):1-12} {WB} {Human}

    [IF={{ 3.266 }}] {Guo LP et al. Smad signaling coincides with epithelial-mesenchymal transition in a rat model of intrauterine adhesion. Am J Transl Res. 2019 Aug 15;11(8):4726-4737. eCollection 2019.} {IHC} {Rat}

    [IF={{ 2.9 }}] {Zhen Liu. et al. Enhanced bacteriostasis and osseointegrative properties of SiRNA-modified polyetheretherketone surface for implant applications. PLOS ONE. 2024 Dec;19(12):e0314091} {WB} {Rat}

    [IF={{ 2.7 }}] {Junfeng He. et al. Effect of the TGF-β/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions. ANIMALS. 2024 Jan;14(14):2072} {WB} {Bovine}

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    文献支持
    phospho-Smad1/5 (Ser463 + Ser465) Rabbit pAb(bs-3418R)-50ul/100ul/200ul
    ￥1180 - 2800