Sample:
Intestine(Mouse) Lysate at 40 ug
Primary: Anti-FBXO45 (bs-13150R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
- ￥1180 - 1980
- Bioss
- bs-13150R
- 2025年10月16日
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-13150R |
| 英文名称 | FBXO45 Rabbit pAb |
| 中文名称 | F-box蛋白45抗体 |
| 英文别名 | F box protein 45; F-box only protein 45; F-box/SPRY domain-containing protein 1; FBSP1_HUMAN; FBX45; fbxo45; hFbxo45. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FBXO45 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 31 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Forms a complex with MYCBP2 and SKP1 but does not appear to form an authentic SCF (SKP1-CUL1-F-box protein) complex (By similarity). Interacts (via B30.2/SPRY domain) with TP73 (via SAM domain); leading to TP73 ubiquitination and degradation by proteasome. Interacts (via SRY domain) with UNC13A; leading to degradation of UNC13A by the proteasome (By similarity). |
| 亚细胞定位 | Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > presynaptic cell membrane. |
| 相似性 | Belongs to the FBXO45/Fsn family. Contains 1 B30.2/SPRY domain. Contains 1 F-box domain. |
| 功能 | Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration (By similarity). Plays a role in the regulation of neurotransmission at mature neurons (By similarity). May controls synaptic activity by controlling UNC13A via ubiquitin dependent pathway (By similarity). Specifically recognizes TP73, promoting its ubiquitination and degradation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Belonging to the F-box family of proteins, FBXO45 is a 286 amino acid protein that contains one C-terminal F-box domain. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein)-type E3 ubiquitin ligase complex and are involved in substrate recognition and protein recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular mechanisms, including the cell cycle, the immune response, signaling cascades and developmental processes. They function by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for degradation by the proteasome after ubiquitination. Via its F-box domain, FBXO45 can directly interact with Skp1 p19 and CUL-1. FBXO45 has been shown to be an estrogen-induced gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-FBXO45 (bs-13150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 30 kDa
Lane 1: Human A431 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-FBXO45 (bs-13150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 30 kDa
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文献和实验该产品被引用文献
[IF={{ 2.8 }}] {Mingxiu Xu. et al.FBXO45 restricts HIV-1 replication by inducing SQSTM1/p62- mediated autophagic degradation of Tat.virology.2025 Feb 12:e0191224.} {} {Human}
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FBXO45 Rabbit pAb(bs-13150R)-50ul/100ul
￥1180 - 1980
