Blank control: A431.
Primary Antibody (green line): Rabbit Anti-NFIB antibody (bs-11899R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-11899R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11899R |
| 英文名称 | NFIB Rabbit pAb |
| 中文名称 | 核因子1B抗体 |
| 英文别名 | CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; NFIB; NFIB_HUMAN; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; NFIB2; NFIB3; HMGIC/NFIB. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NFIB/NF1B2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Other factors Neuroscience > Neurology process > Neurogenesis |
| 亚基 | Binds DNA as a homodimer. |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain. |
| 功能 | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Ovary (Mouse) Lysate at 40 ug
Lane 5: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Ovary (Mouse) Lysate at 40 ug
Lane 5: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:293T Cell (Human) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
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文献和实验该产品被引用文献
[IF={{ 4.4 }}] {Yi Shang. et al. CircIQCH Contributes to the Progression of Breast Cancer by Elevating NFIB Through Decoying miR-139-5p. ENVIRON TOXICOL. 2024 Jun;:} {WB} {Human}
[IF={{ 4.36 }}] {Hanyue Ying. et al. CircATL2 enhances paclitaxel resistance of ovarian cancer via impacting miR-506-3p/NFIB axis. 2021 Sep 19} {WB} {human}
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NFIB Rabbit pAb(bs-11899R)-50ul/100ul/200ul
￥1180 - 2800
