- ￥980 - 4000
- Bioss
- bs-2967R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
100ul/500ul
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 产品编号 | bs-2967R |
| 英文名称 | MBP tag Rabbit pAb |
| 中文名称 | 麦芽糖结合蛋白抗体 |
| 英文别名 | MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI. |
| 产品应用 | WB=1:500-2000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Periplasm. |
| 相似性 | Belongs to the bacterial solute-binding protein 1 family. |
| 功能 | Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:1000-5000} |
100 ng rhC-jun-His-MBP protein (bs-42050P) per lane probed with MBP tag polyclonal antibody respectively, unconjugated (bs-2967R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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文献和实验相关实验
Studies of the Ubiquitin Proteasome System
with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation
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MBP tag Rabbit pAb(bs-2967R)-100ul/500ul
￥980 - 4000
