Sample:
Lane 1: Human HepG2 cell lysates
Primary: Anti-Factor VII heavy chain (bs-4846R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28/51 kDa
Observed band size: 59 kDa
- ￥1180 - 2800
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- bs-4846R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4846R |
| 英文名称 | Factor VII heavy chain Rabbit pAb |
| 中文名称 | 凝血因子7抗体 |
| 英文别名 | Factor VII heavy chain; coagulation factor VII(serum prothrombin conversion accelerator); Coagulation factor VII; Eptacog alfa; F7; FA7_HUMAN; Factor VII; Factor VII light chain; FVII coagulation protein; OTTHUMP00000018733; OTTHUMP00000018734; Proconvertin; Serum prothrombin conversion accelerator; SPCA. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Factor VII heavy chain |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28/51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Coagulation > Extrinsic Cardiovascular > Blood > Serum Proteins |
| 亚基 | Heterodimer of a light chain and a heavy chain linked by a disulfide bond. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Plasma. |
| 翻译后修饰 | The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| 相似性 | Belongs to the peptidase S1 family.
Contains 2 EGF-like domains. Contains 1 Gla (gamma-carboxy-glutamate) domain. Contains 1 peptidase S1 domain. |
| 功能 | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- Factor VII heavy chain (bs-4846R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28/51 kD
Observed band size: 50 kD
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- Factor VII heavy chain (bs-4846R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28/51 kD
Observed band size: 50 kD
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文献和实验该产品被引用文献
[IF={{ 4.307 }}] {Devin Cao. et al. Vascular Endothelial Cells Produce Coagulation Factors That Control Their Growth via Joint Protease-Activated Receptor and C5a Receptor 1 (CD88) Signaling. Am J Pathol. 2022 Feb;192:361} {IF} {Mouse}
[IF={{ 2.93 }}] {Liu, Yang, et al. "A Simple Method for Isolating and Culturing the Rat Brain Microvascular Endothelial Cells." Microvascular Research (2013).} {Other} {="Rat"}
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Factor VII heavy chain Rabbit pAb(bs-4846R)-50ul/100ul/200ul
￥1180 - 2800
